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Blue squares refer to the DB mixtures and metabolites; yellow spheric nodes refer to the predicted objects. PLoS One. 2014; 9(7): e101432. In our previous work, based on the Brownian motion profit ofmalism, we introduced three stochastic perturbation protocols focusinged at improving the PMF calculation with the Jarzynski analogy in removed molecule manipulation experiments and analogous computer simulations.

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